A spatially ordered Fe-Pd bimetallic nanozyme (o-FePd DAN) simulates substrate-induced conformational locking (SCL) through dynamic electronic coupling within the Cl-FeN3C-PdN3 sites. XAFS and DFT studies reveal a bridging structure-driven charge transfer pathway that effectively promotes adaptive regulation and selective catalysis, which is further applied to a POCT visual gene typing platform for HPV.