Nell-1 promotes osteogenic differentiation and mineralization of hPDLSCs and upregulates CYFIP1, Runx2, and CGRP-related signaling; disrupting CYFIP1 affects the osteogenic effects mediated by Nell-1. The CPC-aMF scaffold has a hierarchically micro-fibrous structure, suitable mechanical properties, and degradation behavior, which can protect the seed cells and achieve dual-phase release of Nell-1. In vivo, scaffolds containing hPDLSCs and Nell-1 promote new bone formation, collagen deposition, and defect repair, while also enhancing angiogenesis and nerve ingrowth, with increased markers like CGRP, β3-tubulin, and CD31. Mechanistically, Nell-1 links nerves and osteogenesis through the CYFIP1-MAPK/ERK-Runx2 axis.

Original Source:

Smart Molecules

Pub Date: 2026-07-15

DOI: 10.1002/smo2.70078

Minjia Zhu, Xinyi Li, Jingyi Li, Kan Yu, Zixiang Dai, Le Xiao, Qinrou Zhang,

Zihan Jia, Qingchen Qiao, Zeqing Zhao, Ke Zhang, Yuxing Bai

We focus on customizing functional materials in the field of nanomedicine. According to your experimental needs, we can develop biofunctional nanomaterials in a targeted manner, controlling material structure, particle size, and surface modifications, with a full set of characterization tests. The programs can be repeatedly optimized, with full technical support throughout, supporting research projects related to targeted delivery, bioimaging, and nano-therapy. Feel free to provide your requirements, and we will evaluate a custom plan.

Engineer Zhang: 17715390137
Engineer Chen: 18914047343