He Zhike Anal. Chem, Wuhan University: Using a new method to construct clickable quantum dots to achieve bioorthogonal labeling

Bioorthogonal chemistry is considered to be one of the powerful tools for biomolecular labeling because of its site specificity, mild reaction conditions, high yield, and simple post-processing. At present, people generally use covalent coupling methods to modify commonly used bioorthogonal functional groups (azide or cycloalkynes) on quantum dots (QDs), but this often reduces the quantum yield and stability of QDs, and Increase the hydrated particle size of QDs. In order to overcome these shortcomings, Professor He Zhike of Wuhan University proposed a new method that utilizes the strong interaction between SH and metal ions to prepare two clickable quantum dots.

Key points of this article:

(1) The first system uses azide-DNA functionalized QDs and cyclooctyne (DBCO) modified glucose oxidase (GOx) for biological coupling to form GOx-QDs complexes. After biological coupling, the stability of QDs is improved, while the activity of GOx is effectively enhanced. Studies have shown that the GOx-QDs complex can be used for rapid detection of blood glucose through spectroscopy, naked eye and paper-based analysis equipment.

(2) The second system is DBCO-DNA functionalized quantum dots, which can be used for in-situ bio-orthogonal labeling of HeLa cells through metabolic oligosaccharide engineering. In summary, these clickable QDs based on DNA functionalization are expected to be used to quickly and effectively label biomolecules of interest.

references

Guobin Mao. et al. Novel Method of Clickable Quantum Dot Construction for Bioorthogonal Labeling. Analytical Chemistry. 2020

DOI: 10.1021/acs.analchem.0c03078

https://pubs.acs.org/doi/10.1021/acs.analchem.0c03078

Source of information: Wonders

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