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Temporomandibular joint osteoarthritis (TMJ-OA) is a degenerative disease with limited treatment options. This study constructs manganese-nitrogen-carbon monoelemental nanozymes (Mn-NC SAzymes) - shell demolding composite hydrogel systems, clarifying their Mn-N4 coordination structure, and integrating atomic-level catalytic design and local delivery functions. Leveraging the synergistic dual-enzyme-like activity of the nanozymes and the active repair properties of the shell bacteria, it achieves multi-dimensional synergy in ROS clearance, inflammation regulation, and joint cartilage and bone combined repair. Through molecular mechanism analysis, it clarifies its inhibitory effect on inflammation-induced chondrocyte apoptosis and ECM degradation via the MAPK signaling pathway, and using density functional theory (DFT) to reveal the catalytic reaction pathway. It systematically explains the structure-property relationship of the single-elemental nanozymes and the in vivo functional reconstruction mechanism, promoting the transformation of the treatment mode for TMJ-OA.
01 Research Background
TMJ-OA is a degenerative disease with limited treatment options. During the disease process, excessive reactive oxygen species (ROS) trigger abnormal inflammatory microenvironments, which subsequently lead to chondrocyte apoptosis, ECM degradation, and disorders in cartilage metabolism. This is the core starting point of this study.
02 Main Content
Focusing on the pathological microenvironment of TMJ-OA, the Mn-NC SAzymes - shell demolding composite hydrogel system is designed and constructed. The Mn-N4 coordination structure is determined through X-ray absorption fine structure spectroscopy. This system integrates atomic-level catalytic and local delivery functions. By leveraging the synergistic dual-enzyme-like activity of the nanozymes and the active repair properties of the shell bacteria, it achieves multi-dimensional functional synergy in ROS clearance, inflammation regulation, and joint cartilage and bone combined repair. At the same time, it deeply analyzes the molecular mechanism, clarifying its inhibitory effect on inflammation-induced chondrocyte apoptosis and ECM degradation via the MAPK signaling pathway, and through DFT calculations, revealing the catalytic reaction pathway. It systematically explains the structure-property relationship of the single-elemental nanozymes and the in vivo functional reconstruction mechanism.
03 Research Design
Targeting the ROS-related inflammatory microenvironment of TMJ-OA, the Mn-NC SAzymes - shell demolding composite hydrogel system is designed. The core coordination structure of the material is analyzed through spectroscopic techniques. Combining material science, molecular biology, and theoretical calculation methods, the catalytic performance, regulatory effect on the pathological microenvironment, and bone cartilage repair effect of the composite hydrogel are explored. At the same time, the molecular mechanism and catalytic reaction pathway are clarified.
04 Results
The Mn-NC SAzymes - shell demolding composite hydrogel with the Mn-N4 coordination structure was successfully constructed, achieving effective integration of atomic-level catalytic design and local delivery systems. This material possesses synergistic dual-enzyme-like activity and repair properties, can efficiently clear ROS, regulate the inflammatory microenvironment, inhibit inflammation-induced chondrocyte apoptosis and ECM degradation mediated by the MAPK signaling pathway, and promote joint cartilage and bone combined repair. Through DFT calculations, the catalytic reaction pathway was clarified, and the structure-property relationship of the single-elemental nanozymes and their in vivo functional reconstruction mechanism were systematically explained.
05 Extension of Thoughts
This provides a new direction for the treatment of TMJ-OA from simple symptom relief to multi-dimensional functional reconstruction. It also provides systematic references for the design of the structure, performance optimization, and mechanism research in biomedical applications of single-elemental nanozymes, and offers new ideas for the material intervention strategies for degenerative diseases. 4. Authors: Min Xing, Shuhan Chen, Mengjiao Zhu, Yuanming Cao, Jiayin Feng, Wenhao Qian, Kuicai Ye, Xuanyong Liu, Jiajun Qiu
