PCR product purification kit

It is specially designed for the extraction and purification of PCR products. The surface is modified with a large number of silanol groups. It can specifically bind to the nucleic acid in the solution under high salt and low pH conditions through hydrophobic interaction, hydrogen bonding and electrostatic interaction, and it is equipped with a buffer system. , It can easily recover DNA in PCR products, effectively remove primer dimers, dNTPs, inorganic salts and proteins and other impurities. The whole process is simple and fast to operate, and the PCR products can be purified in 15 minutes. This product has the characteristics of a wide range of recovered DNA fragments, safe and simple operation, high recovery rate, and high DNA purity. It is very conducive to the automation and high-throughput extraction of nucleic acids. The recovered DNA can be directly used for enzymatic digestion, sequencing, PCR reactions and other follow-ups. operate.

■ Product performance

Strong nucleic acid binding capacity: specific surface area>25 m2/g, nucleic acid binding capacity>15ug DNA/mg magnetic beads;

Good operating performance: the magnetic beads are evenly dispersed, have superparamagnetism, the saturation magnetization is 70-80emu/g, and the magnetic response time is less than 30s;

Stability and good batch-to-batch repeatability: uniform particle size and monodisperse, ensuring good results reproducibility;

Good storage stability: complete silicon oxide layer coating, stable use performance, resistance to 6M hydrochloric acid corrosion for more than 5 minutes;

Suitable for a wide range of DNA fragments: 150bp~50kb;

High recovery rate: the recovery rate is as high as 80% or more;

High DNA purity: The recovered PCR product has high purity, A260/280=1.7~1.9, A260/230=1.9~2.3;

The operation is fast and simple: the entire operation process can be completed in 15 minutes without centrifugation;

Applicable to automated, high-throughput PCR product purification;

■ Application direction

PCR product purification;

The recommended dosage of this product is the same volume of magnetic beads as PCR samples;

■ Matters needing attention

1. The magnetic beads are stored in deionized water, freezing, drying and centrifugation may affect the use of magnetic beads;

2. It is normal for the magnetic beads to settle due to gravity. Before using this product, be sure to fully oscillate or ultrasound to keep the magnetic beads in a uniform suspension state;

3. The magnetic beads can be stored stably for 2 years at 2~8℃.