[Product Introduction]
Agarose gel electrophoresis (AGarose) is an important molecular biology technique for the separation and purification of DNA fragments. Magnetic beads DNA gel recovery kit is a NEW DNA gel recovery kit developed by Suzhou Beike Zhenze Biotechnology Co., LTD. This kit kit USES a unique buffer solution in the airport or TBE agarose gel electrophoresis buffer preparation, from dissolving in tile pieces of DNA can be specifically adsorbed onto the surface of the silicon is changed magnetic beads, under the effect of magnetic field, the magnetic beads to carry DNA magnet method for directional movement and gather, separated from the rest of the other impurities completely. Through two simple washes, the impurities can be completely removed. Finally, the DNA combined on the magnetic beads was eluted with water or low-salt buffer. The eluted DNA fragments with high purity and integrity could be directly used for ligating reaction, PCR amplification, DNA sequencing and other molecular biology experiments. The kit can be manually operated, or it can be used for automatic or semi-automatic working platform after parameter debugging of automatic extraction equipment.
【 characteristics 】
L The adsorption amount of magnetic beads is very small, and the repeatability is good.
L Manual or automatic operation is optional.
L Simple operation, no time consuming operation such as centrifugation or filtration.
L High throughput can be achieved
[Preservation methods and Precautions]
Please store the Beads in the kit at 2-8℃ and the rest of the reagents at room temperature. See the packing for the expiry date.
L Freezing and centrifugation are strictly prohibited. Freezing and centrifugation may cause irreversible damage to magnetic beads.
L Magnetic beads will gather into clusters after long-term placement, thus reducing the surface area of magnetic beads and reducing the recovery rate of samples. It is necessary to thoroughly mix magnetic beads by eddy oscillation before use (usually 20 seconds of eddy oscillation).
[Kit composition]
component
100 times
save
MG Buffer
30 ml
The normal temperature
Wash buffer
75% ethanol (for users)
The normal temperature
Elution buffer
5 ml
The normal temperature
bead
2 ml
2-8 ℃
Isopropyl alcohol
Users should bring along their own
The normal temperature
DNA concentration and purity detection :(1) the size of the obtained genomic DNA fragments was related to factors such as sample preservation time and shear force during operation. The concentration and purity of the obtained DNA fragments could be detected by agarose gel electrophoresis and ultraviolet spectrophotometer. (2) DNA should have a significant absorption peak at OD260, and OD260=1 is equivalent to about 50ng/ul double-stranded DNA and 40ng/ul single-stranded DNA. The OD260/OD280 ratio should be 1.7~1.9. If deionized water is used in elution, the ratio will be low, because pH and ions will affect the absorbance, which does not mean low purity.
[Operation Procedure]
The rubber cutting -
1) Quickly cut the target strip from the agarose gel (remove excess agarose gum as far as possible) under the irradiation of long-wave UV lamp, and put it into a clean 1.5mL centrifuge tube after weighing.
The sol -
2) Add a 1:1 volume MG buffer (volume: weight) to the gel (e.g., 100 MG gel with 100 μ L MG buffer). Note: For agarose gel with a concentration greater than 2%, a 2:1 volume of MG buffer was added to the block). The gel mixture was placed at 65 ℃ for 10 min and mixed intermittently until the gel was completely dissolved.
In combination with -
3) Add 20 μ L beads into the centrifuge tube (add after shaking), blow and mix with pipette gun, then stand at room temperature for 3 min, blow and mix once again, and stand for 3 min. Magnetic separation for 20 s, supernatant absorption.
Clean -
4) Add MG buffer of the same volume as that in step 2 into the centrifugal tube, blow and mix with a pipette gun, and then stand at room temperature for 3 min. Magnetic separation was performed on a magnetic rack for 20 s, and the supernatant was absorbed and discarded.
Clean -
(5) Add 700 UL Wash buffer into the centrifugal tube, blow and mix with a pipette gun, then stand at room temperature for about 2 min, place on a magnetic rack for magnetic separation for 20 s, and discard the supernatant. Repeat this step once. The centrifuge tube was inverted for 2-3 min, and then dried at room temperature for 5 min to allow the residual ethanol to volatilize, so as not to affect subsequent experiments.
Des -
6) Add 10-50 ul Elution buffer to the centrifugal tube (Elution buffer is better after preheating at 65-70 ℃, and THE DNA concentration can be increased by reducing the Elution volume or multiple Elution), pipette blowing and mixing for 1-2 min, Magnetic separation was carried out on the magnetic rack for 20 s, then the natant was carefully sucked up and put into the new centrifugal tube. The extracted plasmid DNA could be stored at 2-8 ℃, and the long-term storage should be placed at -20 ℃.
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Warm tip: the products supplied by Beijing Beike Xincai Technology Co., Ltd. are only used for scientific research, not for human body |
| Item ID |
CAS |
ID |
Pack |
Parameter |
Stock |
Make up |
Price |
| BK2021081233 |
|
BK2021081233 |
|
|
100 |
|
$0 |
